Fig. 4: Responsiveness of GNSR genes to boiled culture extracts of leaf microbiota strains.
From: Plant microbiota feedbacks through dose-responsive expression of general non-self response genes
a, Induction levels of the GNSR genes CYP71A12 (top) and AZIL (bottom) by bacterial culture extracts of five leaf microbiota strains two days after treatment exhibit dose dependency. Bacterial suspensions at the indicated densities (x axes, where 1×, 10× and 100× correspond to OD600 of 0.02, 0.2 and 2, respectively, before boiling) were boiled and applied onto A. thaliana seedlings. The data are from n = 2–4 independent experiments (indicated above the x axis), each comprising 18 pooled plants per condition measured in duplicate. b, Induction of CYP71A12 (top) and AZIL (bottom) by bacterial culture extracts at three early time points (3, 24 and 48 h post treatment, indicated above the plots), showing varying dynamics of gene expression between extracts and GNSR genes. Bacterial suspensions of five leaf microbiota strains at an OD600 of 2 (corresponding to an extract density of 100× in a) were boiled and applied onto A. thaliana seedlings. The points indicate mean log2FCs of normalized gene expression relative to axenic control plants. The error bars indicate propagated standard error. The data are from n = 3 independent experiments, each comprising 18 pooled plants per condition measured in duplicate. In a,b, the letters above the points indicate significance levels obtained from one-way ANOVA with Tukey’s post-hoc test.
