Extended Data Fig. 4: Screening microbiota strains with altered abundances in the microbiota of GNSR mutants for CYP71A12 induction potential and plant phenotypes.
From: Plant microbiota feedbacks through dose-responsive expression of general non-self response genes
(A) CYP71A12 induction by nine microbiota strains and the foliar pathogen P. syringae pv. tomato DC3000 (Pst) upon exposure of A. thaliana pCYP71A12::GUS reporter seedlings to bacterial suspensions in a fluorometric assay screen for one day. Included were microbiota strains that were affected by mutations in GNSR components in a microbiota context (highlighted by grey diamonds in Fig. 1b). Vertical plot line indicates mean fluorescence of mock-treated plants on the same plate as the indicated treatment. Boxplot bars indicate median, hinges indicate first and third quartile, whiskers indicate smallest/largest value within 1.5 × IQR (interquartile range). Data from one experiment with n = 6–7 plants per condition (indicated above x-axis). Statistical significance was assessed by two-sided t-test with Bonferroni correction using titer “1×” as reference group (***: P-value ≤ 0.001, **: P-value ≤ 0.01, and *: P-value ≤ 0.05). (B) Representative images of pCYP71A12::GUS reporter plants one day after treatment with indicated bacteria. Pictures were taken immediately before performing the fluorometric assay. (C) Representative images of pCYP71A12::GUS reporter plants three days after treatment (3 d), where disease phenotype caused by Serratia Leaf50 is enhanced. Treatment suspensions of leaf microbiota strains or Pst were prepared at a 10× titer. (A, B) Indicated titers of 1×, 10×, and 100× correspond to bacterial treatment suspensions adjusted to an OD600 of 0.002, 0.02, and 0.2 before treatment, respectively.
