Fig. 1: Amborella and its genome structure.

a,b, Female (a) and male (b) Amborella flowers. c,d, The Amborella genome (c) and chromosome 9 (Chr09, d) are typical of flowering plants: gene-rich chromosome arms and repeat-dense, large pericentromeric regions. Gene positions were extracted from the protein-coding gene annotations, repeats from EDTA and exact matches of 536,985 female-specific k-mers (W-mers). Syntenic mapping was calculated using AnchorWave and processed using SyRI, only plotting inversions, insertions and deletions >10 kb. Visualization of synteny was accomplished with GENESPACE and sliding windows with gscTools. The sex-determination region of Chr09 with W-mers is highlighted in d. All chromosomes in haplotype 1 and all but four in haplotype 2 have both left and right telomeres in the assembly (flagged with red *), defined as a region of ≥150 bp made up of ≥90% plant telomere k-mers (CCCGAAA, CCCTAAA, RC) separated by no more than 100 bp. CDS, coding sequence.