Extended Data Fig. 4: Relative expression and activity of Tau class glutathione reductases (GSTs) in the presence of DNAN.
From: New weapons explosive exhibits persistent toxicity in plants
(a) Upregulation of GSTs from a transcriptomics study of two-week-old, liquid culture grown plants dosed with DNAN for 6 hours (n = 5, mean ± SD). Statistical analysis was performed by one-way ANOVA and Tukey’s post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. HPLC analysis of the conjugating activity of recombinant GST: (b) U1, (c) U2, (d) U3, (e) U7, (f) U9, (g) U11, and (h) U22 to GSH. Both DNAN and TNT were tested. TNT-conjugate peaks are labelled on the trace as previously identified7,8. No peaks were present in DMSO controls or DNAN substrate reactions. HPLC analysis was repeated on three separate reactions. All the data and p values can be found in the Source data file.
