Extended Data Fig. 4: Differential ABA-triggered subcellular Ca²⁺ dynamics within 50 s.

Ca²⁺ signals of of CRS-PM, CRS, CRS-NLS within 50 s in response to ABA in the epidermal cell of the root meristem zone in 7-day-old transgenic plants. The signals were subtracted from the mock control presented in Fig. 3b–d. CRS-PM detected the ABA-induced Ca²⁺ transient first but the nuclear Ca²⁺ oscillation revealed by CRS-NLS did not start until 100 s (Fig. 3d).