Extended Data Fig. 7: CRS, CRS-NLS and CRS-PM show similar Ca2+ dynamic range in root tip cells. | Nature Plants

Extended Data Fig. 7: CRS, CRS-NLS and CRS-PM show similar Ca2+ dynamic range in root tip cells.

From: ABA-activated low-nanomolar Ca2+–CPK signalling controls root cap cycle plasticity and stress adaptation

Extended Data Fig. 7: CRS, CRS-NLS and CRS-PM show similar Ca2+ dynamic range in root tip cells.

Range of ABA-induced changes in cytosolic [Ca2+]. The highest ratio represents the maximum [Ca2+] with 1mM CaCl2 in digitonin-treated cells for 20 minutes [Ca2+] in the root tip. The lowest ratio represents the minimum [Ca2+] in the BAPTA-AM and EGTA-treated cells for 15 minutes in the root tip. Error bars denote ±s.e.m., data from at least three independent experiments (total number of plants: CRS (EGTA and BAPTA-AM), n=9; CRS (Digitonin), n=9; CRS-NLS (EGTA and BAPTA-AM), n=9; CRS (Digitonin), n=10; CRS-PM (EGTA and BAPTA-AM), n=7; CRS-PM (Digitonin), n=7). Ratio, relative fluorescence ratio of GCaMP6s to dTomato. All experiments were conducted in at least three biological repeats with similar results.

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