Extended Data Fig. 3: The CRS variants do not exhibit overt growth defect phenotypes. | Nature Plants

Extended Data Fig. 3: The CRS variants do not exhibit overt growth defect phenotypes.

From: ABA-activated low-nanomolar Ca2+–CPK signalling controls root cap cycle plasticity and stress adaptation

Extended Data Fig. 3: The CRS variants do not exhibit overt growth defect phenotypes.

a, WT, CRS, CRS-NLS and CRS-PM transgenic plants were grown in soil for 21 days. Scale bars, 1 cm. b,c,d,e, Resembling WT, expression of the CRS variants did not alter plant growth. Fresh weight (b), primary root length (c) and leaf area (d) of 7-day-old Arabidopsis seedlings were measured when grown on 1/2 MS solid medium. e, CRS, CRS-NLS, CRS-PM, and WT plants were grown in soil for statistical flowering time measurements. Error bars denote ±s.e.m., data from at least three independent experiments (total number of plants: WT, n=28; CRS, n=23; CRS-NLS, n=25; CRS-PM, n=24 (b), WT, n=28; CRS, n=26; CRS-NLS, n=27; CRS-PM, n=25 (c), WT, n=16; CRS, n=16; CRS-NLS, n=16; CRS-PM, n=16 (d), WT, n=30; CRS, n=30; CRS-NLS, n=30; CRS-PM, n=30 (d)). ns (not significant) P > 0.05, (statistical significance determined by two-tailed non-paired Student’s t test). All experiments were conducted in at least three biological repeats with similar results.

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