Extended Data Fig. 4: Lysozyme enhances LDH30 uptake by tomato early bicellular pollen.
A, Representative confocal images of early bicellular tomato pollen showing that a 4 h pre-treatment with 1 mg/ml lysozyme enhances uptake of uncoated LDH30 nanoparticles loaded with fluorescein. B, Flow cytometry data showing enhanced uptake of LDH30 labelled with fluorescein (LDH30-FL) by early bicellular pollen after lysozyme pre-treatment (Lysozyme then LDH30-FL) or lysozyme coating of the nanosheets (Lys@LDH30-FL). C, Flow cytometry data showing pre-treatment or nanosheet coating with heat treated lysozyme (HT-Lysozyme/HT-Lys) failed to enhance uptake of LDH30-FL by pollen. The fluorescence intensity was normalized based on the auto-fluorescence of pollen in the untreated control group. Treatments labelled with the same letter in B and C were not significantly different (p < 0.05) based on one-way ANOVA with post-hoc Tukey’s HSD test. Data in B and C are presented as the mean ± SEM for n = 3 biological replicates.
