Extended Data Fig. 3: Lysozyme enzyme activity is required for enhancing LDH30 uptake.
A, Representative confocal images of N. benthamiana roots showing that a 4 h pre-treatment with 1 mg/ml of imidazome-inhibited lysozyme failed to enhance uptake of uncoated LDH30 nanoparticles loaded with fluorescein (LDH30-FL), and comparison to roots pre-treated with lysozyme or treated with lysozyme-coated LDH nanoparticles (Lys@LDH30-FL). Bar = 200 µm. B, Quantitative fluorescence analysis showing pre-treatment with imidazole-inhibited lysozyme did not enhance uptake of LDH30-FL (mean ± SEM for n = 9 biological replicates). Treatments labelled with the same letter in B were not significantly different (p < 0.05) based on one-way ANOVA with post-hoc Tukey’s HSD test.
