Extended Data Fig. 2: Detection of native NSP1 by anti-NSP1 antibody and CRISPR–Cas9 gene editing of NSP1a. | Nature Plants

Extended Data Fig. 2: Detection of native NSP1 by anti-NSP1 antibody and CRISPR–Cas9 gene editing of NSP1a.

From: The BRUTUS iron sensor and E3 ligase facilitates soybean root nodulation by monoubiquitination of NSP1

Extended Data Fig. 2: Detection of native NSP1 by anti-NSP1 antibody and CRISPR–Cas9 gene editing of NSP1a.The alternative text for this image may have been generated using AI.

a, Western blot of native NSP1a abundance in nodules of WT and nsp1a-1 mutant using anti-NSP1 antibody. The arrow indicates the specific band of NSP1a and NSP1b. The experiment was repeated for three times with similar results. b, Schematic of the NSP1a gene. The nsp1a-1 mutant in the Wm82 background has a 10-bp (540–549 bp) deletion in the coding region induced by CRISPR–Cas9. The red font in the mutant represents the new translational stop codon due to a frame shift arising from Cas9 nuclease activity. c, Diagram of wild-type and mutant NSP1a protein. d, Sanger sequencing chromatograms of wild-type Wm82 and nsp1a-1 mutant. The blue box indicates deleted region in the nsp1a-1 mutant.

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