Extended Data Fig. 5: Identification and analysis of the replacement in wheat mutants at the site of VRT-A2. | Nature Plants

Extended Data Fig. 5: Identification and analysis of the replacement in wheat mutants at the site of VRT-A2.

From: Precise deletion, replacement and inversion of large DNA fragments in plants using dual prime editing

Extended Data Fig. 5: Identification and analysis of the replacement in wheat mutants at the site of VRT-A2.The alternative text for this image may have been generated using AI.

a, Schematic representation of the replacement of 567-bp sequence with 157 bp induced by DualPE. Spacers are shown in orange and green for pegL and pegR, respectively. RT templates encoding insertions marked as 3’ flap are shown in purple and blue, and blue and pink for pegL and pegR, respectively. Homologous overlap between pegL and pegR is shown in blue. The original 560 bp sequences are highlighted in blue, and the additional 7 bp flanking sequences that were deleted for PAM design are highlighted in red. The desired 157 bp insertion sequences are shown in purple, blue, and pink. b, Sanger sequencing chromatograms of T0-2, T0-8, T0-12, and T0-14. c, Segregation analysis of T0-2 and T0-8 lines derived from DualPE-mediated replacement. HO, homozygous; HE, heterozygous; WT, wild type.

Back to article page