Extended Data Fig. 10: Input and output page of the dual-pegRNA designing webserver. | Nature Plants

Extended Data Fig. 10: Input and output page of the dual-pegRNA designing webserver.

From: Precise deletion, replacement and inversion of large DNA fragments in plants using dual prime editing

Extended Data Fig. 10: Input and output page of the dual-pegRNA designing webserver.The alternative text for this image may have been generated using AI.

a, The input page of DualPE-Finder webserver includes the input textbox, user-defined parameters and primer design. b, Output page of DualPE-Finder webserver. The webserver displays all possible candidate pegLs and pegRs, providing details on spacer, PBS, RTT, primers and sequence after editing. Candidates are recommended based on the cumulative distance of pegL nicking from the start position and pegR nicking from the end position of the desired editing fragments, specifically ranked from the shortest to the longest distance.

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