Extended Data Fig. 3: Genetic basis of tonoplast ATG8ylation.
From: ATG8ylation of vacuolar membrane protects plants against cell wall damage
(a) Confocal micrographs of GFP-ATG8A expressed in atg2 mutant background of Arabidopsis thaliana root cells treated with Torin (1.5 hours, 9 µM) or ES20-1 (8 hours, 100 µM). Each panel includes a single optical slice and a corresponding bright field image to illustrate ATG8A behavior. Representative image from 10 seedlings analyzed under each treatment. Scale bar, 10 µm. (b) Confocal micrographs of GFP-ATG8F or GFP-ATG8I expressed in wild-type or atg4 mutant background of Arabidopsis thaliana root cells treated with Torin (1.5 hours, 9 µM) or ES20-1 (8 hours, 100 µM). Each panel includes a single optical slice and a corresponding bright field image to illustrate ATG8A behavior. Representative image from 10 seedlings analyzed under each treatment. Scale bar, 10 µm.
