Extended Data Fig. 7: Fusion of MADS31 to eGFP can function in plants. | Nature Plants

Extended Data Fig. 7: Fusion of MADS31 to eGFP can function in plants.

From: MADS31 supports female germline development by repressing the post-fertilization programme in cereal ovules

Extended Data Fig. 7: Fusion of MADS31 to eGFP can function in plants.The alternative text for this image may have been generated using AI.

a, Extreme dwarfism in Ubi::MADS31 transgenic plants. 40 days and 120 days indicate growth time after plants were transferred to soil. Scale bars, 1 cm. b, Nucellus patterning in wild-type (WT) and pro::MADS31-eGFP ovules. Left, toluidine blue stained longitudinal sections. Right, LM19 labelled demethylesterified pectin in the cell walls of nucellus. Scale bars, 25 μm (Ov3) and 100 μm (Ov10). c, Ratios of inner nucellus area versus outer nucellus area in WT, mads31 and pro::MADS31-eGFP ovules. d, Corrected total cell fluorescence (CTCF) of LM19 immunosignals in the nucellus of WT, mads31 and pro::MADS31-eGFP ovules. e, Various degrees of dwarfism in pro::MADS31-eGFP transgenic plants. Scale bar, 10 cm. f, Flag leaf inclination in wild-type (WT) and pro::MADS31-eGFP plants. Scale bar, 2 cm. g, Expression of MADS31-eGFP fusion protein in three transgenic mads31 plants. Scale bars, 100 μm. h, Mature spikes of WT, mads31 and mads31/pro::MADS31-eGFP plants. Yellow asterisks indicate sterile spikelets. Scale bar, 2 cm. i, Longitudinal sections of wild-type (WT), mads31 and mads31/pro::MADS31-eGFP ovules. Scale bars, 50 μm. j, Ratios of inner nucellus area versus outer nucellus area in WT, mads31 and mads31/pro::MADS31-eGFP ovules. In c, d and j, the data are shown as mean ± s.d.; n = 5 ovules; t-test for unpaired two-sample data, two-sided.

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