Fig. 5: The inhibition of TSWV L by ribavirin triphosphate.
a, Effects of ribavirin triphosphate treatment on TSWV infection. N. benthamiana plants inoculated with TSWV were subjected to treatment with either buffer or ribavirin triphosphate (100 μg ml−1). Left: disease development was photographed at 14 dpi. Right: the accumulation level of TSWV NP protein in the systemic leaves of treated plants was determined via immunoblot analysis using an NP-protein-specific antibody. Ponceau S staining of the RuBisCO large subunit was used to indicate sample loading. b, Left: cut-away global view of the TSWV L–ribavirin triphosphate complex (PDB ID: 9J8V). The L protein is coloured by domain with the same colour code as in Fig. 1. Ribavirin triphosphate is represented as a blue shape with a dashed outline. The right panel shows detailed interactions between L and ribavirin triphosphate. The hydrogen bonds are shown as yellow dashed lines.
