Extended Data Fig. 2: Screening of kinases that phosphorylate BSR1 protein. | Nature Plants

Extended Data Fig. 2: Screening of kinases that phosphorylate BSR1 protein.

From: A cell wall-associated kinase phosphorylates NLR immune receptor to negatively regulate resistosome formation

Extended Data Fig. 2: Screening of kinases that phosphorylate BSR1 protein.The alternative text for this image may have been generated using AI.

a, List of seven kinases selected from the proximity labeling data for subsequent screening. b, BiFC assay to test the interaction between BSR1 and seven candidate kinase proteins. Scale bars, 20 ÎĽm. c, Immunoblot analysis to detect the protein expression in the infiltrated leaves shown in (b). The asterisk represents a nonspecific band. d, Analysis of the kinase activity of each protein using the universal kinase substrate myelin basic protein. e, f, Analysis of the phosphorylation of BSR1 by six candidate kinases. Autoradiographic images are shown on the top and the CBB-stained gel used as a loading control are shown below.

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