Extended Data Fig. 5: Identification of phosphorylation sites within BSR1 proteins driven by its native promoter. | Nature Plants

Extended Data Fig. 5: Identification of phosphorylation sites within BSR1 proteins driven by its native promoter.

From: A cell wall-associated kinase phosphorylates NLR immune receptor to negatively regulate resistosome formation

Extended Data Fig. 5: Identification of phosphorylation sites within BSR1 proteins driven by its native promoter.The alternative text for this image may have been generated using AI.

a, Diagram of the BSR1 expression vector under its native promoter with different lengths. b, Immunoblot analysis of BSR1 expression driven by its native promoter. Asterisk represents a nonspecific band. c, Cell death phenotype of transiently expressed BSR1 under its native promoter in the presence of BSMV. Leaf regions infiltrated with Agrobacterium carrying an empty vector (EV) served as the control. d, Silver staining analysis of immunoprecipitation-enriched BSR1 protein expressed under the native promoter. e, Protein sequence coverage of the LC-MS/MS analysis of the enriched BSR1 protein shown in panel d, red letters indicate the peptides that were identified by LC-MS/MS. f, LC-MS/MS spectra demonstrate that Ser470 of BSR1 expressed under its native promoter is phosphorylated in vivo.

Source data

Back to article page