Extended Data Fig. 10: Analysis of the kinase activity of WAKL20 and its variants.
a, Schematic representation of WAKL20 and its variants used in the in vitro phosphorylation assay. SP, signal peptide; TM, transmembrane domain. b, Kinase activity of WAKL20 and its variants. c, Kinase activity of WAKL20 or WAKL20Δ26-262 in the presence or absence of BSMV infection. For panels b and c, the in vitro phosphorylation assay was conducted using proteins immunoprecipitated from N. benthamiana leaves transiently expressing WAKL20 or its variants, as indicated above each panel, with 5 μg of universal substrate Myelin basic protein (MBP). EXP. 1–3 represents three independent biological replicates. d and e show the quantification of the Myelin basic protein band intensity from panels b and c, respectively. Error bars represent the mean ± SD (n = 3 biological repeats). Different letters in the chart signify statistically significant differences among different groups, determined through unpaired two-tailed t-test (P < 0.05; see the Source Data file for the exact P values). n.s. = not significant, P > 0.05. No adjustments for multiple comparisons were made for indication of the significance.
