Extended Data Fig. 6: Phosphomimetic mutations of TIR repress TIR-mediated cell death.
From: TIR immune signalling is blocked by phosphorylation to maintain plant growth
Cell death phenotypes of wild-type and mutated TIRs upon transient expression in N. benthamiana leaves. The cell-death phenotype was visualized under visible light (left panel) or UV (right panel) and imaged 3 days after agroinfiltration inoculation. Different degrees of cell death were defined as the cell death score (0, blue; 1, coral pink; 2, orange). Each color on stacked bars shows the proportions (in numbers) of the corresponding degrees of cell death. a, Expression of SNC1TIR-YFP, non-phosphorylatable SNC1TIR-S18A-YFP, and phospho-mimic SNC1TIR-S18D-YFP. Plant leaves were infiltrated with Agrobacterium tumefaciens bacteria with OD600 at 0.2 (left) or 0.4 (right). b, Expression of SNC1TIR-YFP, non-phosphorylatable SNC1TIR-S18A/S26A (AA)-YFP, phospho-mimic SNC1TIR- S18D/S26D (DD)-YFP, and catalytically dead SNC1TIR-E93A-YFP. c, Expression of AbTIR-YFP, non-phosphorylatable AbTIR-S140A-YFP, phospho-mimic AbTIR-S140D-YFP, and catalytically dead AbTIR-E208A-YFP. All experiments were repeated at least three times with similar results.
