Extended Data Fig. 10: Regulation of SARM1TIR by TBK1.
From: TIR immune signalling is blocked by phosphorylation to maintain plant growth
a, Expression of hTBK1-Flag and Actin in N. benthamiana leaves transiently expressing hSARM1TIR-GFP with or without hTBK1-Flag was analyzed by reverse transcription PCR. The anti-GFP antibody was used to detect hSARM1TIR-GFP proteins. b, Human SARM1TIR-GFP-Flag and hSARM1TIR-S567A-GFP-Flag proteins were transiently expressed in the NRK1-HEK293T or TBK1/NRK1-HEK293T stable cell lines and detected by western blot. The anti-Flag antibody was used to detect wild-type and mutated hSARM1TIR-GFP-Flag. The anti-Myc antibody was used to detect TBK1-Myc. The anti-actin was used as a loading control. All experiments were repeated at least three times with similar results.
