Extended Data Fig. 4: Detection of virus and Cas9/sgRNA in M1 progeny seedlings through RT-PCR and immunoblotting.
From: Transgene- and tissue culture-free heritable genome editing using RNA virus-based delivery in wheat
a, Detection of Cas9-T mRNA, sgRNATaPDS-T and BYSMV genomic RNA in M1 progeny seedlings by RT-PCR. The M0 infected and mock wheat leaves served as positive and negative controls, respectively. TaActin served as an endogenous control. The experiment was repeated in M1 seedlings three times. b, Detection of the Cas9 and BYSMV M proteins in M0 infected wheat plants and M1 progeny seedlings by immunoblotting. GAPC2 served as protein loading control. The experiment was repeated in M1 seedlings three times.
