Extended Data Fig. 8: Localization of CR proteins and HEI10 in scep3, spo11-1-3 and mtopVIB-2 as well as no direct interaction of SCEP3 with selected proteins in Y2H.
Immunolocalization of a, ZYP1-C and HEI10, b, SCEP1 and HEI10, and c, SCEP2 and HEI10 in early pachytene nuclei of WT and scep3-1. d, Quantification of SCEP3 and HEI10 foci numbers in scep3-1 (HEI10, 91 ± 8.1; n = 7), spo11-1-3 (HEI10, 17 ± 5.6; SCEP3, 18 ± 3.6; n = 15), and mtopVIB-2 (HEI10, 38 ± 9.7; SCEP3, 39 ± 8.1; n = 14) as well as their percentage of overlap in spo11-1-3 and mtopVIB-2. Data are presented as mean ± standard deviation (s.d.). e, Immunolocalization of SCEP3 and HEI10 in early pachytene nuclei of spo11-1-3 and mtopVIB-2. DAPI-stained DNA in gray. Scale bar, 10 µm. f, Interactions tested between SCEP3 and axis(-associated) (ASY4, COMET or PRD3) or ZMM proteins (ZIP4, HEI10, MER3 or PTD) in Y2H. TDO (SD/-LTH) is a less stringent and QDO (SD/-LTHA) is a more stringent medium used for selection. The experiments (a-c, e) were repeated at least three times with similar results.
