Extended Data Fig. 3: Expression pattern and subcellular localization of KOIN.
From: A receptor–kinase cascade confers cold-induced root growth inhibition in Arabidopsis
a, Tissue specific expression of KOIN in Col-0 based on Genevestigator data. Expression potential (%) indicates average expression across tissues relative to the highest expression sample (set as 100%). b, GUS staining of pKOIN::GUS transgenic plants. c, RT–qPCR analysis of KOIN expression in Col-0 under cold treatment. Data represent mean ± s.d. (n = 3), representative of three independent experiments. d, Genevestigator-based expression patterns of KOIN under cold stress. e, Subcellular localization of KOIN-GFP in various tissues of koin-2 KOIN complementation plants. GFP, GFP fluorescence; PI, propidium iodide staining (red); merged images show overlays with chlorophyll, PI, or FM4-64. Scale bars: 10 μm (root tip, meristem zone, hypocotyl, epidermal cells); 5 μm (elongation zone, root hairs). f, KOIN-GFP localization in root tip. Scale bar, 20 μm. g, KOIN protein levels in Col-0 under cold stress. Anti-KOIN and anti-Actin antibodies were used. h, Identification of Super::KOIN-GFP (KOIN-OE) transgenic plants in Col-0 and crpk1-1 background. i-j, KOIN protein levels in Col-0 (i) or crpk1-1 (j) backgrounds under cold. In (h-j), anti-GFP and anti-Actin antibodies were used.
