Extended Data Fig. 4: Re-localization of KOIN-GFP from BFA compartments to the plasma membrane is impaired in crpk1-1 seedlings.
From: A receptor–kinase cascade confers cold-induced root growth inhibition in Arabidopsis
a, Quantification of KOIN-GFP fluorescence intensity in Col-0 and crpk1-1 roots under cold treatment, corresponding to Fig. 3a–d. b, Relative fluorescence intensity of KOIN-GFP during recycling assays, corresponding to Fig. 3g, h. c, Number of BFA bodies per cell after sequential CHX, BFA + CHX, and BFA washout treatments as described in Fig. 3g, h. d, Quantitation of plasma membrane and cytosol fluorescence intensities of KOIN-GFP in Col-0 and crpk1-1 roots under the same treatments. Data are mean ± s.d. (n = 3 biological replicates). Different letters indicate significant differences (P < 0.05, one-way ANOVA with Tukey’s test). Exact P values are provided in the Supplementary Table 2.
