Extended Data Fig. 6: Identification of KOIN complemented transgenic plants.
From: A receptor–kinase cascade confers cold-induced root growth inhibition in Arabidopsis
a, Immunoblot analysis of KOIN, KOINS314A and koin-2 KOINS314E expression in koin background under the native promoter. KOIN was detected with anti-GFP antibodies; Actin serves as a loading control. b, In planta phosphorylation of wild-type and S314A mutant KOIN after cold treatment. Immunoprecipitated KOIN-GFP and KOINS314A-GFP were analyzed by immunoblotting using anti-GFP and anti-phosphoserine antibodies. c,d, Root length of 5-d-old Col-0, koin-2, koin-2 KOINS314A and koin-2 KOINS314E seedlings grown at 22 °C and then maintained at 22 °C (c) or transferred to 4 °C (d) for 10 days. Data represent mean ± s.d. (n = 20, one biological replicate), representative of three independent experiments. Different letters indicate statistically significant differences (P < 0.05, one-way ANOVA with Tukey’s post hoc test). Exact P values are provided in the Supplementary Table 2.
