Extended Data Fig. 5: Phenotypic and protein level analysis of gte2/7, cdkl9 mutants and rescued plants. | Nature Plants

Extended Data Fig. 5: Phenotypic and protein level analysis of gte2/7, cdkl9 mutants and rescued plants.

From: Bromodomain-containing proteins interact with a non-canonical RNA polymerase II kinase to maintain gene expression upon heat stress

Extended Data Fig. 5

a-d. Vann diagrams show the overlapped genes with decreased Ser2P (a) or Ser5P (c) and increased Ser2P (b) or Ser5P (d) in gte2/7 or cdkl9 under normal condition. e. The seed germination rate of WT, gte2/7 and GTE7/gte2gte7 on CK, 200 mM NaCl, 350 mM Mannitol and 4°C. Data are presented as mean ± s.e.m. (n = 3 biological replicates). f. The seed germination phenotypes of WT, gte2/7 and GTE7/gte2gte7 under heat stress. g. Quantifications of the relative germination rate for each genotype presented in (f) after heat stress. Data are presented as mean ± s.e.m. (n = 12 biological replicates). P values are calculated by two-tailed Student’s t test. h, i. Vann diagrams show the overlapped genes with increased Ser2P (h) or Ser5P (i) in gte2/7 or cdkl9 under heat condition. j. Protein levels of GTE2-HA, GTE7-HA and CDKL9-HA under normal and Heat conditions. ACTIN served as control. The experiments were repeated independently two times and showed similar results.

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