Extended Data Fig. 7: Chromatin associations of GTE2, GTE7 and CDKL9. | Nature Plants

Extended Data Fig. 7: Chromatin associations of GTE2, GTE7 and CDKL9.

From: Bromodomain-containing proteins interact with a non-canonical RNA polymerase II kinase to maintain gene expression upon heat stress

Extended Data Fig. 7

a, b. Chromosomal views of GTE2 and GTE7 under normal (a) and heat (b) condition distribution on five chromosomes. The Y-axis represents the log2 value of GTE2-HA and GTE7-HA ChIP-seq reads relative to untagged WT control. Chr1, Chr2, Chr3, Chr4 and Chr5 represent chromosomes 1 to 5, respectively. Black triangles indicate the location of centromeric regions. c. Metaplots and heatmaps show the distribution patterns of GTE2 and GTE7 protein on all genes. TSS, transcription start site; TES, transcription end site; −2 and 2 kb represent 2 kb upstream of TSS and 2 kb downstream TES, respectively. d. Additional biological replicate for Fig. 5p showing ChIP-quantitative PCR (ChIP-qPCR) analysis for CDKL9 enrichment under normal and heat condition on representative genes (n = 2 technical replicates). e, f. ChIP-quantitative PCR (ChIP-qPCR) analysis of CDKL9 enrichment under normal and heat condition on representative genes chosen from the GTE2 and GTE7 bound genes (n = 2 technical replicates). e and f represent two biological replicates.

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