Extended Data Fig. 6: Characterization of the negative effect of R723H on flg22Rso recognition and the enhanced recognition capability of the ‘superGmFLS2’ variant.
a–c, Effects of the R723H mutation on flg22Pa- and flg22Rso-induced ROS production (n = 12 leaf discs). d–g, Comparison of ROS burst kinetics (d), total flg22Pa-induced ROS production (e), flg22Pa- and flg22Rso-induced cytoplasmic calcium influx (f, n = 16 leaf discs), and protein expression levels (g) between ‘superGmFLS2’ and GmFLS2b (related to Fig. 4g). For line charts, data are presented as mean ± SEM. For box plots: center line, median; box limits, upper and lower quartiles; whiskers, 1.5 times interquartile range; points, outliers. Two-tailed Mann-Whitney test was performed for b, c, and e. Similar results were obtained from three independent experiments.
