Extended Data Fig. 4: ROS burst kinetics and total flg22^Pa-induced ROS production of GmFLS2a/b chimeras and GmFLS2a variants with predicted gain-of-recognition polymorphic residues.

a, ROS burst kinetics corresponding to Fig. 3b. b–d, ROS burst kinetics (b), total flg22^Pa-induced ROS production (c), and protein expression levels (d) of GmFLS2a/b chimeras (related to Fig. 3c). e, Protein expression levels of chimera ‘abbb’ at varying agroinfiltration OD₆₀₀ values. f, g, Flg22^Pa- and flg22^Rso-induced ROS production kinetics (f) and total ROS production (g) of chimera ‘abbb’ at varying agroinfiltration OD₆₀₀ values (n = 72 leaf discs). h, Flg22^Pa- and flg22^Rso-induced ROS responses of ‘aabb’ and ‘aaba’ chimeras (n = 12 leaf discs). i, j, ROS production kinetics (i), and total flg22^Pa-induced ROS production (j) of GmFLS2a variants carrying predicted gain-of-recognition residues (related to Fig. 3g, n = 12 leaf discs). For line charts, data are presented as mean ± SEM. For box plots: center line, median; box limits, upper and lower quartiles; whiskers, 1.5 times interquartile range; points, outliers. Kruskal-Wallis test followed by Dunn’s multiple comparisons test was used for statistical analysis of c, g, and j. ‘n.s.’ indicates no significant difference. See Source data for exact P values. Similar results were obtained from two (d–g) or three (a–c, h–j) independent experiments.

Source data