Extended Data Fig. 3: SUVH1 binds DNA methylation in the ROS1 promoter and promotes ROS1 expression.
From: Plants repress ROS1 expression to attenuate heat-induced transposon burst
a, ChIP-qPCR assay analyzing the binding of SUVH1 at the ROS1 locus. The upper schematic illustrates the positions of primers (P1 - P3), and the lower column chart depicts the enrichment of SUVH1 at the ROS1 locus. Data represent mean ± s.d., n = 3 biological replicates. Two-tailed Student’s t-test. b, Quantification of SUVH1 transcript levels in Col-0 and SUVH1-Flag transgenic plants under control and heat-stress conditions. Data represent mean ± s.d., n = 3 biological replicates. Two-tailed Student’s t-test. c, Western blot analysis showing protein levels of SUVH1 under control (C) and heat-stress (H) conditions. ACTIN protein was used as the loading control. d, Quantification of ROS1 transcript levels in Col-0 and suvh1 suvh3 under control and heat-stress conditions. Data represent mean ± s.d., n = 3 biological replicates. Two-way ANOVA with Bonferroni’s multiple comparisons test.
