Extended Data Fig. 5: Transport analysis of RE1.
From: RETICULATA1 is a plastid-localized basic amino acid transporter
(a) Representative scheme of the transport assay. Isolated mitochondrial membranes (b) were reconstituted (1) into liposomes (a) with or without 20 mM substrate (c). External substrate was removed via size-exclusion chromatography (2), and the transport reaction was initiated by diluting the proteoliposomes in transport mix (3) containing 0.2 mM substrate (orange triangle) and radioactive labeled substrate (orange star). Samples were taken (4) after 2, 4, 8, 16, 32 and 64 min and the radioactivity in the proteoliposomes was determined by liquid scintillation counting. (b–f) Orn uptake of RE1 of five independent biological replicates. Proteoliposomes were preloaded with 20 mM Orn. Data are shown as mean ± SD of technical triplicates.
