Fig. 5: De novo basic amino acid biosynthesis is altered in the re-6 mutant after Lys treatment.
From: RETICULATA1 is a plastid-localized basic amino acid transporter
a–f, Relative responses of the absolute amounts of labelled Arg (a), Citr (b), Lys (c), Orn (d), Asp (e) and Gln (f) in 14-day-old Col-0 and re-6 seedlings 2 days after labelling with 4 mM 15NH4Cl. Data are shown as mean ± s.d. Different letters indicate statistically significant differences between means (P < 0.05; one-way ANOVA with Tukey’s test). g–i, The percentage of unlabelled and labelled Orn (g), Citr (h) and Arg (i). Metabolites were extracted from the green tissue of 14-day-old seedlings, 2 days after labelling with 4 mM 15NH4Cl with (orange triangles) or without (black circles) 1 mM Lys. Asterisks indicate statistically significant differences (n.s., not significant; ***P < 0.001; two-sided Student’s t-test). Exact P values (a–i) are shown in the Source data with 95% confidence intervals. j,k, Schematic depictions of the impact of Lys treatment on de novo Arg, Citr and Orn biosynthesis in the re-6 mutant (j) and the wild type (k). Reduced biosynthesis of Arg, Citr and Orn in the re-6 mutant due to a feedback inhibition in their biosynthetic pathway (red line) caused by an accumulation of Arg (red arrow) as a result of a knockout of RE1 (white circle with red cross) and an inhibition of RER1 (grey circle) transport activity by excess Lys (orange triangles) is emphasized by a reduced line thickness. KG, α-Ketoglutarate.
