Extended Data Fig. 2: Characterization of Arabidopsis hug1 and hug2 mutants.
From: Kinesins control male germ unit assembly for sperm delivery in Arabidopsis
(a) Genomic structure (top) and summary of the hug1 mutations generated by, EMS mutagenesis or CRISPR–Cas9 (bottom). (b) Genomic structure (top) and summary of the hug2 mutations generated by CRISPR–Cas9 (bottom) or T-DNA insertions. (c-d) Seed set in siliques of WT and hug1 and hug2 single and double mutants. Scale bar, 1 mm. (e-f) Quantification of seed set shown as in (c–d). In (e), n = 3,282, 3,701, 3,479, 3,493, 4,081 and 3,537 ovules for genotypes from left to right. Data are the mean ± S.D. In (f), n = 1,523, 1,546, 1,621, 1,554, 1,601 and 1,479 ovules. Data are the mean ± S.D. Two-sided Students’ t-test, ***P = 2.31 × 10−5 (hug1-2 hug2-2) and ***P = 6.88 × 10−6 (♀ WT × ♂ hug1-2 hug2-2) compared with WT. (g) Pollen-germination rate for WT and hug double mutants. Data are the mean ± S.D. Two-sided Students’ t-test, n.s. non-significant. P = 0.9268 n = 300 grains for each sample. (h–i) Successful pollen tube entry into the ovule in WT (h), and hug1-2 hug2-2 (i) double mutants. Asterisks indicate ovules penetrated by more than one pollen tube. Arrows indicate micropyles wherein the pollen tube enters the embryo sac. Scale bar, 100 μm.
