Fig. 5: Short homozygous blocks interrupting the heterozygous region suppress local crossover stimulation. | Nature Plants

Fig. 5: Short homozygous blocks interrupting the heterozygous region suppress local crossover stimulation.

From: Enhancing local meiotic crossovers in Arabidopsis and maize through juxtaposition of heterozygous and homozygous regions

Fig. 5: Short homozygous blocks interrupting the heterozygous region suppress local crossover stimulation.The alternative text for this image may have been generated using AI.

a, Interhomologue SNP distribution in the M(ws3,lg1) × M cross along chromosome 2. Grey vertical bars represent SNP density per 10 kb, with the burgundy trend line showing SNP density per 1 Mb. Grey horizontal bars beneath the graph indicate estimated heterozygous regions based on SNPs. Coloured lines mark the positions of the ws3–lg1 interval used to measure Rf. The navy blue square on the x axis shows the centromere location. The inset offers a close-up of the interval, with the SNP density plots below partially trimmed. b, Rf (%) measured by segregation of ws3–lg1 markers in F2 generations for Hybrid M(ws3,lg1) × Ku(R1) and Juxtaposed M(ws3,lg1) × M crosses (male/female averaged). The two-sided P values were estimated by Welch’s t-test; ****P = 7.1 × 10−5. The numbers of individuals are indicated below the box plots. Each data point represents measurements from one plant. Different colours within the same group indicate data collected in different years. The centre line of the box plot shows the median, and the upper and lower bounds show the 75th and 25th percentiles. c,d, As in a and b, but for the M(lg1,gl2) crosses. ****P = 1.3 × 10−5. e, As in a, but for the Hybrid M(ws3,gl2) × Ku(R1) and Interrupted juxtaposed M(ws3,gl2) × M crosses, where the heterozygous region contains a short homozygous block (gap). f, As in b, but measured by segregation of ws3–gl2 markers. NS, not significant. g, The effect of genetic structure on Rf. Heterozygous regions are shown as light-grey shaded areas in otherwise homozygous chromosome. The intervals used for recombination measurements are indicated by coloured dashed lines, and the average Rf in these intervals for full hybrids is represented by the grey bars. Small violet arrows denote expected subsegmental crossover changes based on homozygous/heterozygous state. Crossover frequency is lower when heterozygosity is interrupted by homozygosity (compare chromosome configurations 1 and 2).

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