Extended Data Fig. 6: The interactions between RAKs and RopGEF6/7.
From: RLCKs phosphorylate RopGEFs to control auxin-dependent Arabidopsis development
a and b, Luciferase complementation assays displayed the interactions of RAK1/2/3/4 with RopGEF6 (a) and RopGEF7 (b). Circles indicate areas that were infiltrated with Agrobacterium containing the indicated constructs. c, MBP-tagged RAKs pull down GFP-RopGEF6 from 7-day-old transgenic plants harboring RopGEF6p:GFP-RopGEF6. Lower panel shows total plant proteins stained with CBB. MBP was used as a control. d and e, Both GST-tagged RAK1/3 (d) and MBP-tagged RAK2/4 (e) pull down YFP-RopGEF7 from 9-day-old transgenic plants harboring 35Spro:YFP-RopGEF7. Lower panel shows total plant proteins stained with CBB. GST in (d) or MBP in (e) was used as a control. The experiments in c–e were performed independently three times with similar results.
