Extended Data Fig. 8: Phosphatase treatment of immunoprecipitated RopGEF1-GFP.
From: RLCKs phosphorylate RopGEFs to control auxin-dependent Arabidopsis development
Phosphatase treatment of immunoprecipitated RopGEF1-GFP extracted from 7-d old 35S:RopGEF1-GFP transgenic plants caused migration shift in gradient SDS-PAGE gel. Immunoprecipitated RopGEF1-GFP protein was treated with lambda (Ī») phosphatase for 1āh. Anti-GFP was used to detect RopGEF1-GFP. The experiments were performed at least three times with similar results.
