Extended Data Fig. 9: Wortmannin treatment causes RopGEF1-GFP proteins sequestered to large intracellular aggregates in the root cells of rak4m seedlings.
From: RLCKs phosphorylate RopGEFs to control auxin-dependent Arabidopsis development
a, Confocal images showed the subcellular distribution of GFP-labeled RopGEF1 in the root epidermis cells of meristem and elongation zones of 4-day-old wild type (upper panel) and rak4m (lower panel) treated with 0.1% (v/v) DMSO (wortmannin solvent) or 20āĪ¼M wortmannin for 3āh. Scale bars=20 Ī¼m. b, Immunoblot analysis of RopGEF1-GFP protein levels in wortmannin-treated and untreated rak4m seedlings. Total protein was isolated from 7-day-old rak4m transgenic seedlings carrying RopGEF1pro:RopGEF1-GFP treated without and with 20āĪ¼M wortmannin for 3āh. RopGEF1-GFP proteins were probed with GFP antibody and ponceau S staining was used as a loading control. The experiments in a and b were performed at least three times with similar results.
