Extended Data Fig. 10: Guanine nucleotide exchange activity of ROP6 were enhanced by RopGEF1 in a dosage-dependent manner.
From: RLCKs phosphorylate RopGEFs to control auxin-dependent Arabidopsis development
a, The activity of the GEF domain of Dbs for a human Cdc42 protein were detected as a positive control by a fluorescence spectroscopy-based assay. Results are representative of three independent assays that gave similar results. b, Data showed dosage-dependent guanine nucleotide exchange activity of RopGEF1 for ROP6. Various concentrations (0, 0.5, 1, 2āĪ¼M) of RopGEF1 were tested toward ROP6. The experiments in a and b were performed independently at least three times with similar results.
