Extended Data Fig. 2: Additional Reporters and selection markers.
a, Calibration curve for purified NanoLuc concentration (ng) versus luminescence intensity (a.u.). The relationship between nanoluciferase abundance and luminescence is linear. b, Fluorescent reporter characterization. Fold over WT fluorescence is displayed for each strain, normalized by chlorophyll content, each expressing a different fluorescent reporter. nbiological = 16. c, PCR analysis of tobramycin-resistant strains. The first section of the gel illustrates PCR products of the tobramycin resistance gene, exclusive to the five genetically modified strains, with ~1500 bp bands, absent in WT. The latter section shows PCR products from the insertion site, with ~8000 bp bands in engineered strains and a 2000 bp band in WT, indicating homoplasmy in the modified strains. d, Selection marker for chloroplast transformation, with two replicates and two negative controls for each one. Successful selection after chloroplast transformation can be observed. e, Western blot analysis of transplastomic strains producing mScarlet HIS-tagged, Strep-tagged, Hibit-tagged in the Cterm position. Each strain is also tested for the presence of mScarlet using mScarlet specific probes. Ponceau gels are shown for each gel, each experiment was performed twice to confirm reproducibility. All box plots show median as solid line, the box with lower and upper quartile, and the whiskers representing the data points that fall within 1.5 times the interquartile range (IQR) from the lower and upper quartiles. Any data point outside this range is considered as outlier.ss.
