Extended Data Fig. 8: Phenotype associated to amiR-mail2 lines. | Nature Plants

Extended Data Fig. 8: Phenotype associated to amiR-mail2 lines.

From: Plant mobile domain protein–DNA motif modules counteract Polycomb silencing to stabilize gene expression

Extended Data Fig. 8

(a) Examples of developmental phenotype recovered from 10-day-old in vitro growing plants. (b) MAIL2 transcript accumulation in WT and amiR-mail2_4 and _5 lines, detected by RT-qPCR using primers framing the amiRNA-targeted cutting site. Data are normalized to ACT2 and further normalized to WT; values represent means from three biological replicates ± s.e.m and statistical significance for differences to the WT are indicated (unpaired, two-sided Student’s t-test). (c) Photos of five-week-old WT plants compared to amiR-mail2_4 mutant plants containing a single transgene locus at heterozygous (he) or homozygous (ho) state. (d) Two-week-old phenotypes of WT, amiR-mail2 and gMAIL2-1-9xMYC-complemented amiR-mail2 seedlings. Scale bar is 1 cm.

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