Fig. 7: CPSF30-L and ECT12 cooperatively suppress the transcription and abundance of m6A retrotransposon RNAs.
From: RNA m6A regulates the transcription and heterochromatin state of retrotransposons in Arabidopsis
a, Box plot showing the expression levels of m6A retrotransposon RNAs in WT, cpsf30-l, ect12-3 and cpsf30-l ect12 seedlings. The medians (horizontal lines), interquartile ranges (boxes), and ±1.5× the interquartile range (whiskers) of the data are shown (n = 139 m6A retrotransposon RNAs). P values were determined using paired two-sided Wilcoxon tests. b, Venn diagram of upregulated m6A retrotransposon RNAs upon the depletion of individual m6A readers and double m6A readers. c,d, Relative transcriptional (c) and expression levels (d) of COPIA22 and ATRE1 in the indicated 6-day-old seedlings. ACTIN2 is used as a negative and internal control. The data are shown relative to that in the WT. The data are shown as mean ± s.e.m. (n = 3 biological replicates). The P values are from two-tailed Student’s t-tests. e, Hypothetical model depicting the mechanism of m6A-mediated heterochromatin silencing of retrotransposons in Arabidopsis. m6A on retrotransposon RNAs, deposited by m6A writers, is recognized by CPSF30-L/ECT12 reader proteins, of which CPSF30-L recruits SUVH4/5/6 and ATXR5/6 to achieve repressive H3 K9 dimethylation and H3 K27 monomethylation, leading to closed heterochromatin and reduced transcription. Panel e created with BioRender.com.
