Despite the extensive use of Arabidopsis thaliana as a model system, parts of its biology remain unknown, including the architecture of its largest cellular protein assembly, the nuclear pore complex (NPC). Using cryo-electron tomography, we have obtained the three-dimensional architecture of the A. thaliana NPC, which suggests it has both conserved and distinct features.
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References
Tamura, K., Fukao, Y., Iwamoto, M., Haraguchi, T. & Hara-Nishimura, I. Identification and characterization of nuclear pore complex components in Arabidopsis thaliana. Plant Cell 22, 4084 (2010). This paper identifies most of the nucleoporins in A. thaliana.
Pöge, M. et al. Making plant tissue accessible for cryo-electron tomography. Reviewed preprint at https://doi.org/10.7554/eLife.106455 (2025). This publication reports a protocol enabling cryo-TEM from plant tissue.
Sanchez Carrillo, I. B., Hoffmann, P. C., Barff, T., Beck, M. & Germain, H. Preparing Arabidopsis thaliana root protoplasts for cryo electron tomography. Front. Plant Sci. 14, 1261180 (2023). This paper reports a protocol that enabled cryo-TEM on samples from plant cells and was used in this study.
Mosalaganti, S. et al. AI-based structure prediction empowers integrative structural analysis of human nuclear pores. Science 376, eabm9506 (2022). This paper includes the model of the human NPC and describes the approach we used to generate the A. thaliana model for comparison with the human model.
Mosalaganti, S. et al. In situ architecture of the algal nuclear pore complex. Nat. Commun. 9, 2361 (2018). This paper reports the architecture of the C. reinhardtii NPC.
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This is a summary of: Sanchez Carrillo, I. B. et al. In situ architecture of the nuclear pore complex of the higher plant Arabidopsis thaliana. Nat. Plants https://doi.org/10.1038/s41477-025-02138-y (2025).
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The Arabidopsis thaliana nuclear pore complex structure reveals conserved and distinct features. Nat. Plants 11, 2198–2199 (2025). https://doi.org/10.1038/s41477-025-02139-x
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DOI: https://doi.org/10.1038/s41477-025-02139-x
