Extended Data Fig. 4: SAIR1 interacts and functions together with SAIR2.
a, Bacterial growth determined at 3 d post-spray inoculation with Pst DC3000 (n = 8). The box plots show the median (centre), the first and third quartiles (bounds of the box), and the whiskers extend to the furthest data point.b, Phenotypes of 4-week-old indicated genotypes. Scale bar, 1 cm. c, Representative confocal images of guard cells from pSAIR1::SAIR1-YFP-1/sair1-1 at 1 h post-spray inoculation with mock or Pst DC3000. Scale bar, 2 μm. d, Representative confocal images of guard cells from SAIR2-YFP-OX2 in Col-0 or fls2 background at 1 h post-spray inoculation with mock or Pst DC3000. Scale bar, 5 μm. e, SAIR1 specifically forms condensates in guard cells. Leaves of SAIR1-YFP-OX1 were used. Scale bar, 50 μm (left panel), and 5 μm (right panel). f, Representative confocal images of guard cells from SAIR2-YFP-OX2 at 30 min post-infiltration with mock, nlp20, elf18 or chitin. Scale bar, 5 μm. g, Stomatal apertures upon treatment with mock, nlp20, elf18, or chitin for 1 h. Sample sizes are indicated. h, Phenotypes of 4-week-old Col-0 and SAIR2 overexpression plants (SAIR2-YFP-OX1 and -OX2). SAIR2 overexpression causes mild autoimmune phenotypes. Scale bar, 1 cm. i, Representative confocal images of guard cells from SAIR2-YFP-OX1 at 1 h post-spray inoculation with mock or Pst DC3000. Scale bar, 5 μm. j, Co-immunoprecipitation (Co-IP) assays showing the SAIR1-SAIR1 and SAIR1-SAIR2 interactions. IB, immunoblot. k, Yeast two-hybrid assays showing the SAIR1-SAIR1 and SAIR1-SAIR2 interactions. Individual data points are presented with means ± s.d. (g). n = independent biological replicates (a). Different letters indicate significant differences by one-way ANOVA with Tukey’s multiple comparisons test (P < 0.05) (a, g). The exact P values are provided in the Source Data file (a, g). The experiments were repeated three times with similar results (b-k).
