Extended Data Fig. 5: Condensation of SAIR1 is required for stomatal immunity.
a, PrLD is essential for Pst DC3000-induced SAIR2 condensation. Scale bar, 20 μm.b, Protein expression of SAIR1-YFP or its variants in the indicated Arabidopsis transgenic lines. Ponceau S staining served as a loading control. c, Images of stomatal apertures of the indicated genotypes upon treatment with mock or Pst DC3000 for 1 h. Red lines show profiles of guard cells. Scale bar, 50 μm. d, Representative confocal images of guard cells from pSAIR1::SAIR1ΔIDR-FUS-YFP-1/sair1-1 at 1 h post-spray inoculation with mock or Pst DC3000. Scale bar, 5 μm. e, Phenotypes of 4-week-old plants of the indicated genotypes. Scale bar, 1 cm. f, Stomatal apertures at 1 h post treatment with mock or Pst DC3000. Sample sizes are indicated. g, Pathogen entry assays showing luminescent bacteria in leaves after 1 h exposure to Psm ES4326-lux. h, Bacterial growth determined at 3 d post-spray inoculation with Pst DC3000 (n = 8). The box plots show the median (centre), the first and third quartiles (bounds of the box), and the whiskers extend to the furthest data point.Individual data points are presented with means ± s.d. (f). n = independent biological replicates (h). Different letters indicate significant differences by one-way ANOVA with Tukey’s multiple comparisons test (P < 0.05) (f, h). The exact P values are provided in the Source Data file (f, h). The experiments were repeated three times with similar results (a-f, g).
