Extended Data Fig. 2: Mutants, overexpression plants, and expression of SAIR1.
a, Schematics of SAIR1 and SAIR2. T-DNA insertion sites are indicated by triangles. b, GUS staining of abaxial leaf epidermal cells of 4-week-old pSAIR2::GUS plants. Scale bar, 20 μm. c, Phenotypes of the indicated genotypes. SAIR1 overexpression causes autoimmune dwarfism. Scale bar, 1 cm. d, Relative expression of SAIR1 in 4-week-old Col-0, sair1-1, and SAIR1 overexpression plants (SAIR1-YFP-OX1 and OX2) (n = 3). e, Relative expression of SAIR2 in 4-week-old Col-0 and sair2 plants (n = 3). f, Relative expression of SAIR1 in 4-week-old Col-0 and sair1-2 plants (n = 3). g, Stomatal apertures upon treatment with mock, Pst DC3000, or flg22 for 1 h. Sample sizes are indicated. h, Pathogen entry assays showing luminescent bacteria in leaves after 1 h exposure to Psm ES4326-lux. i, Bacterial growth determined at 3 d post-spray inoculation with Pst DC3000 (n = 8). j,k, Relative expression of SAIR1 in 4-week-old Col-0 plants under mock or Pst DC3000 treatment for 24 h (j), or under mock or flg22 treatment for 1 h (k) (n = 3). l, Bacterial growth determined at 3 d post-infiltration inoculation with Pst DC3000 (OD600nm = 0.0001) (n = 8). Individual data points are presented with means ± s.d. (d-g, j, k). n = independent biological replicates (d-f, i-l). The box plots show the median (centre), the first and third quartiles (bounds of the box), and the whiskers extend to the furthest data point (i, l). Data were analyzed by one-way ANOVA with Tukey’s multiple comparisons test (P < 0.05) (d, g and l) or two-tailed t-test (e,f, i-k). The exact P values are provided in the Source Data file (d, g, l). The experiments were repeated three times with similar results (b, c, g, h).
