Fig. 2: SCEP3 localizes to meiotic chromosomes at prophase I.
From: SCEP3 initiates synapsis and implements crossover interference in Arabidopsis
a–i, Co-immunofluorescence of SCEP3N antibody (cyan) with ASY1 (magenta) in wild-type (a–c), scep3-2−/− (d–f) and scep3-2+/− (g–i) Arabidopsis meiotic prophase I chromosome spreads at leptotene, zygotene and pachytene. The arrows in the white box in a show small (i) and large (ii) foci, and SCEP3 foci localized at the SC central element preceding a synaptic fork are indicated by the white box in b. j–l, Co-immunofluorescence of SCEP3C antibody (cyan) with ASY1 (magenta) in wild-type Arabidopsis meiotic prophase I chromosome spreads at leptotene (j), zygotene (k) and pachytene (l). m–r, Immunolocalization of SCEP3 to recombination and SC protein mutants varies in foci number. Co-immunofluorescence of ASY1 or REC8 (magenta) with SCEP3N (cyan) in spo11-1, dmc1, hei10, asy1, asy3 and zyp1 homozygous mutant meiotic prophase I chromosome spreads is shown. Representative images are shown from a sample of 50 cells from each genotype. DNA is stained with DAPI (blue), apart from hei10. Scale bars, 10 µm.
