Fig. 3: Interaxial distances measured at pachytene in the wild type and at the pachytene-like stage in the asynaptic scep3−/− mutant.
From: SCEP3 initiates synapsis and implements crossover interference in Arabidopsis
a, Immunolocalization of chromosome axis protein ASY1 (magenta) and SCEP3 (cyan) in the wild type and scep3−/− showing an example of axis width measurement sampling (yellow transect in the white inset box). Representative images are shown from a sample of three cells from each genotype. Scale bars, 10 µm (left). b, Profile plots of examples from the interaxial measurements showing inter-peak distance. c, Comparison of interaxial distances in the wild type and scep3-2 (the data are presented as mean ± s.d., n = 3 cells, 20 measurements taken from each cell). The asterisks indicate significant differences (****P < 0.0001, two-tailed Mann–Whitney U-test). d–r, ZYP1 localized as foci during zygotene in scep3−/− (d–f), while in scep3-2+/− ZYP1 polymerization completes but SCEP3 loading is intermittent (g–l). PCH2 co-localizes with SCEP3N during pachytene in wild type, while in scep3−/− only a few PCH2 foci are observed (m–r). Panels d–f show co-immunofluorescence of ZYP1N (blue) with ASY1 (magenta) on scep3−/− meiotic prophase I chromosomes at zygotene. Panels g–l show co-immunofluorescence of SCEP3N (yellow) and ZYP1 (blue) with ASY1 (magenta) on wild type (g–i) and scep3-2+/− (j–l) meiotic prophase I chromosome spreads at leptotene, zygotene and pachytene. The white boxes in k and l highlight the intermittent SCEP3 signal relative to the complete ZYP1 signal. Panels m–r show co-immunofluorescence of SCEP3N and ASY1 (yellow) with PCH2 (magenta) in wild type (m–o) and scep3-2−/− (p–r) meiotic prophase I chromosome spreads at leptotene, zygotene and pachytene. Intermittent PCH2 and SCEP3 signal in scep3-2−/− can be observed in the white box in r. Representative images are shown from a sample of 50 cells from each genotype. Scale bars, 10 µm.
