Extended Data Fig. 1: Characterization of T-DNA integration and additional expression information on AKT2var plant lines.
From: Engineering vascular potassium transport increases yield and drought resilience of cassava
(A) Schematic representation of the mutant AtAKT2 gene (S210N-S329N, AKT2var) fused to the Arabidopsis AKT2 promoter that was transformed into cassava. (B) Southern Blot analysis of AKT2var transgenic lines (AKT2var-4255, 4261, 4262, 4264, 4265 and 4266) compared to a wild type (WT) plant. (C) Semi-quantitative real-time PCR measurements of relative AKT2var mRNA expression levels in source leaf tissue (SoL), petiole (Pet), upper stem peel (USP) and core (USC), lower stem peel (LSP) and core (LSC), and root peel (RP) and core (RC) with the corresponding MeGAPDH loading control of AKT2var-4266. The PCR was performed with 35 cycles. (B-C) Molecular marker is Gene Ruler 1 kb DNA Ladder (Thermo Fischer Scientific, Waltham, Massachusetts, USA). Primers “AKT2_CDS_RT_F1/R1” were used (see Methods section; ‘RT–PCR analysis’).
