Fig. 1: JISS1 expression is induced systemically by ETI.
a, Luciferase activity in JISS1::LUC plants following DCavrRpm1, DC, DChrpA or mock (MgCl2) challenges at 4:30 hpi. Throughout the figure, white asterisks indicate infiltrated leaves, and red carets indicate leaves used for signal intensity analysis (d). The images are false-coloured by signal intensity, as indicated by individual calibration bars. b, Temporal spatial dynamics of luciferase activity in JISS1::LUC plants following DCavrRpm1 challenge, initiating at 3 hpi. The 3:20 hpi, 3:50 hpi and 4:30 hpi images capture the systemic spread of the signal over time. c, Different Avr genes display temporal specificity in the activation of systemic JISS1::LUC: DCavrRpm1 (4 hpi), DCavrRps4 (13:20 hpi) and DCavrRpt2 (15:20 hpi), compared with the DChrpA control. d, JISS1::LUC signal intensity in leaves adjacent to infiltration (red carets in c) plotted over time (h). e, Fv/Fm is strongly suppressed during ETI following DCavrRpm1 (3 hpi), DCavrRpt2 (10:00 hpi) or DCavrRps4 (16:00 hpi) challenge compared with DChrpA. f, Visualization of Fv/Fm suppression wherein orange indicates Fv/Fm of a healthy leaf (~0.8), green represents a reduction in Fv/Fm as ETI progresses (~0.6) and blue represents a strong impact of ETI on Fv/Fm (~0.3). Representative images of over ten repeats are shown.
