Extended Data Fig. 1: Schematic of the JISS1:LUC construct and its T-DNA integration location in the Arabidopsis genome. | Nature Plants

Extended Data Fig. 1: Schematic of the JISS1:LUC construct and its T-DNA integration location in the Arabidopsis genome.

From: Rapid local and systemic jasmonate signalling drives the initiation and establishment of plant systemic immunity

Extended Data Fig. 1: Schematic of the JISS1:LUC construct and its T-DNA integration location in the Arabidopsis genome.

(A). The Photinus pyralis LUC2P reporter gene (from Promega pGL4-11) was PCR amplified and cloned into pCAMBIA1302 digested with Kpn1 and Pml1 to generate pC1LUCP with a NOS terminator. A 1651 bp JISS1 fragment containing the promoter and encoding the first 84aa of JISS1 was PCR amplified as described64 generating a hygromycin selectable JISS1:LUC construct. (B) Eight independent homozygous lines A. thaliana Col-5 JISS1:LUC expressing transgenic lines were generated and tested for luciferase expression in systemic leaves following an immunising challenge with DCavrRpm1. A homozygous line showing strong JISS1:LUC expression was chosen and the genomic location of the JISS1:LUC T-DNA construct was determined at position 18270147 in the coding region of AT4G39240.1 sequence analysis as per70.

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